One rational approach to comparing multiple samples is to concatenate all samples together, creating a new keyword. (Links to Workspace Template, FCS files, v9 tSNE Tutorial). In such a case, the Population Comparison platform can concatenate all of the control samples in order to use the average of all the control samples for. A use case tutorial with example data and workspace template is available. In FlowJo, the Population Comparison platform is used to compare single or multiple parameters from a test sample to a single control sample OR a composite of more than one control sample. To familiarize yourself with this interface, read this chapter. The following pages will describe and illustrate the process of running tSNE in FlowJo v9. Once you have a compensation matrix and wish to examine it, you will be using the Matrix Editor. If you acquired your data with single-stain controls and wish to compensate it using FlowJo, read this chapter. Click Concatenate on the bottom right, and wait for the new FCS file to be created. CHAPTER 2 – Creating a FlowJo compensation matrix from single stain controls If, for any reason, you want to alter the matrix values, read this chapter. Opening Sample and Other Data Files: FCS Files: FlowJo accepts FCS files from nearly all cytometers. This keyword is read and data in the file are compensated when the data is read into FlowJo. FlowJo accepts a variety of file types in addition to Flow Cytometry Standard (FCS) files. WebNote that FlowJo DOES NOT save your FCS. Many data files contain their own pre-defined compensation matrix keyword, which was written when the file was created. morphine oral solution pil FlowJo: how do I avoid SampleID overlapping after i concatenate. CHAPTER 1 – Altering an acquisition matrix Poorly scaled data will effect downstream compensation, so get into the habit of adjusting transforms through the “T” button in FlowJo’s graph window, prior to opening the compensation wizard for single stained control assignment. Note, Compensation should always be preceded by scaling your data appropriately. For your convenience, this document is broken into chapters. This page will help acquaint you with the various ways to interact with Compensation Matrices in FlowJo.
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